The Lyme Disease Network
Medical / Scientific Abstract
|Title:||PCR detection of Borrelia burgdorferi DNA in cerebrospinal fluid of Lyme neuroborreliosis patients [see comments]|
|Authors:||Keller TL, Halperin JJ, Whitman M|
|Source:||Neurology 1992 Jan;42(1):32-42|
|Organization:||Division of Cell and Molecular Biology Dana Farber Cancer Institute Boston MA.|
We used the polymerase chain reaction (PCR), a method useful in the detection of Borrelia burgdorferi in vitro, to evaluate CSF in patients thought to have neuroborreliosis. Nested pairs of oligonucleotide primers were designed to recognize the C-terminal region of B burgdorferi OspA. CSF samples were obtained from (1) patients with immunologic evidence of systemic B burgdorferi infection and clinical manifestations suggestive of CNS dysfunction, (2) seronegative patients with clinical disorders consistent with Lyme borreliosis, and (3) patient and contamination controls; all were analyzed in a blinded fashion. PCR detected B burgdorferi OspA DNA in CSF of (1) 10 of 11 patients with Lyme encephalopathy, (2) 28 of 37 patients with inflammatory CNS disease, (3) seven of seven seronegative patients with Lyme-compatible disorders, and (4) zero of 23 patient controls. Zero of 83 additional contamination controls were PCR-positive. In eight patients from whom we obtained CSF before and after parenteral antimicrobial therapy, PCR results invariably predicted clinical outcome accurately.
Antibody Formation, Base Sequence, Borrelia burgdorferi, GE, Central Nervous System Diseases, CF, Cerebrospinal Fluid, IM, DNA, Viral, CF, Human, Lyme Disease, CF, Molecular Sequence Data, Oligonucleotide Probes, GE, Polymerase Chain Reaction, Support, Non-U.S. Gov't, Medline File, Cancerlit File
Unique ID: 92131307
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